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Overview
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Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S11P family of ribosomal proteins. It is located in the cytoplasm. Transcript variants utilizing alternative transcription initiation sites have been described in the literature. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. In Chinese hamster ovary cells, mutations in this gene can lead to resistance to emetine, a protein synthesis inhibitor. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene.
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- Properties
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Overview
Immunohistochemistry of paraffin-embedded mouse brain using RPS14 antibody (STJ28810) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of MCF7 cells using RPS14 antibody (STJ28810). Blue: DAPI for nuclear staining.
Western blot analysis of extracts of various cell lines, using RPS14 antibody (STJ28810) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.
Anti-RPS14 antibody
Anti-RPS14 antibody
Catalog Number:
A001036875STJ
Mfr. No.:
STJ28810
Price:
$297
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