Name: Anti-ROBO1/DUTT1 antibody
Price: 499.00 USD

- Overview
  - Please contact us at for specific academic pricing.
- Properties
  - Host
    
    goat
    
    Antibody Type
    
    polyclonal
    
    Reactivity
    
    human; mouse; rat; dog; cow
    
    Purification
    
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    
    Conjugate
    
    Unconjugated
    
    Formulation
    
    Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
    
    Storage
    
    Store at -20°C and avoid freeze-thaw cycles.
    
    Concentration
    
    0.5 mg/ml
    
    Regulatory Status
    
    RUO
    
    Antigen
    
    Roundabout Homolog 1; Deleted In U Twenty Twenty; H-Robo-1 (Gene ID:6091; UniProt:Q9Y6N7)
    
    * For research use only
- Applications
  - Application
    
    ELISA; WB; IHC; IF; Flow Cytometry
    
    Application Description
    
    ELISA-antibody detection limit dilution 1:8000.
    WB-An anonymous source observed an approx 250kDa band in Human Endothelial cell lysates at 1ug/ml antibody concentration, thought to be due to glycosylation. Some proteolytic breakdown products were also seen. Primary incubation 1 hour at room temperature.
    IHC-Paraffin embedded Human Liver. Recommended concentration: 2µg/ml.
    IF-Strong expression of the protein seen in the Golgi and membranes of HeLa cells. Recommended concentration: 10µg/ml.
    FC-Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.