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STJ70319 (1µg/ml) staining of HepG2 (A) and Jurkat (B) nuclear cell lysate. (35µg protein in RIPA buffer). Detected by chemiluminescence.

STJ70319 Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) fol

Anti-RNF8 antibody

Anti-RNF8 antibody

Catalog Number:

A001042129STJ

Mfr. No.:

STJ70319

Price:

$499

Size:

100 µg

Quantity:

Add to Cart:

- Overview
  - Please contact us at for specific academic pricing.
- Properties
  - Host
    
    goat
    
    Antibody Type
    
    polyclonal
    
    Reactivity
    
    human
    
    Purification
    
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    
    Conjugate
    
    Unconjugated
    
    Formulation
    
    Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
    
    Storage
    
    Store at -20°C and avoid freeze-thaw cycles.
    
    Concentration
    
    0.5 mg/ml
    
    Regulatory Status
    
    RUO
    
    Antigen
    
    E3 Ubiquitin-Protein Ligase Rnf8; Hrnf8; Ring Finger Protein 8; Ring-Type E3 Ubiquitin Transferase Rnf8 (Gene ID:9025; UniProt:O76064)
    
    * For research use only
- Applications
  - Application
    
    ELISA; WB; Flow Cytometry
    
    Application Description
    
    ELISA-antibody detection limit dilution 1:128000.
    WB-Approx 55kDa band observed in Human Testis and Lung lysates and approx. 50kDa in nuclear lysates of cell lines HepG2 and Jurkat. Recommended concentration 0.5-2µg/ml. Primary incubation 1 hour at room temperature.
    IF-
    FC-Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.

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