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Overview
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The elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proteins proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also named activator 1, is a protein complex consisting of five distinct subunits of 140, 40, 38, 37, and 36 kD. This gene encodes the 37 kD subunit. This subunit forms a core complex with the 36 and 40 kDa subunits. The core complex possesses DNA-dependent ATPase activity, which was found to be stimulated by PCNA in an in vitro system. Alternatively spliced transcript variants encoding the same protein have been reported.
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- Properties
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Overview
Immunofluorescence analysis of U2OS cells using RFC4 antibody (STJ27438). Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 200ug extracts of K562 cells using 1ug RFC4 antibody (STJ27438). Western blot was performed from the immunoprecipitate using RFC4 antibody (STJ27438) at a dilition of 1:1000.
Immunofluorescence analysis of U2OS cells using RFC4 antibody (STJ27438). Blue: DAPI for nuclear staining.
Immunohistochemistry of paraffin-embedded human breast cancer using RFC4 Antibody (STJ27438) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat testis using RFC4 Antibody (STJ27438) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat testis using RFC4 Antibody (STJ27438) at dilution of 1:100 (40x lens).
Western blot analysis of extracts of various cell lines, using RFC4 antibody (STJ27438) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.
Anti-RFC4 antibody
Anti-RFC4 antibody
Catalog Number:
A001035897STJ
Mfr. No.:
STJ27438
Price:
$297
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