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Overview
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This gene product is highly similar to Schizosaccharomyces pombe rad9, a cell cycle checkpoint protein required for cell cycle arrest and DNA damage repair. This protein possesses 3' to 5' exonuclease activity, which may contribute to its role in sensing and repairing DNA damage. It forms a checkpoint protein complex with RAD1 and HUS1. This complex is recruited by checkpoint protein RAD17 to the sites of DNA damage, which is thought to be important for triggering the checkpoint-signaling cascade. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
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Overview
Immunofluorescence analysis of MCF-7 cells using RAD9A antibody (STJ25283). Blue: DAPI for nuclear staining.
Immunohistochemistry of paraffin-embedded rat liver using RAD9A antibody (STJ25283) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human liver damage using RAD9A antibody (STJ25283) at dilution of 1:200 (40x lens).
Western blot analysis of extracts of various cell lines, using RAD9A antibody (STJ25283) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.
Anti-RAD9A antibody
Anti-RAD9A antibody
Catalog Number:
A001034860STJ
Mfr. No.:
STJ25283
Price:
$297
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