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Overview
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Pre-mRNA splicing occurs in 2 sequential transesterification steps. The protein encoded by this gene is a component of both U2-and U12-dependent spliceosomes, and found to be essential for the catalytic step II in pre-mRNA splicing process. It contains several WD repeats, which function in protein-protein interactions. This protein has a sequence similarity to yeast Prp8 protein. This gene is a candidate gene for autosomal dominant retinitis pigmentosa.
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Overview
Immunofluorescence analysis of U-251MG cells using PRPF8 antibody (STJ27847) at dilution of 1:100 (20x lens). Blue: DAPI for nuclear staining.
Immunohistochemistry of paraffin-embedded mouse stomach using PRPF8 antibody (STJ27847) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human esophagus using PRPF8 antibody (STJ27847) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using PRPF8 antibody (STJ27847) at dilution of 1:100 (40x lens).
Western blot analysis of extracts of various cell lines, using PRPF8 antibody (STJ27847) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.
Anti-PRPF8 antibody
Anti-PRPF8 antibody
Catalog Number:
A001036144STJ
Mfr. No.:
STJ27847
Price:
$297
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