Name: Anti-POLRMT antibody
Price: 380.00 USD

- Overview
  - Please contact us at for specific academic pricing.
- Properties
  - Host
    
    goat
    
    Antibody Type
    
    polyclonal
    
    Reactivity
    
    human
    
    Purification
    
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    
    Conjugate
    
    Unconjugated
    
    Formulation
    
    Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
    
    Storage
    
    Store at -20°C and avoid freeze-thaw cycles.
    
    Concentration
    
    0.5 mg/ml
    
    Regulatory Status
    
    RUO
    
    Antigen
    
    Dna-Directed Rna Polymerase-Mitochondrial; Mtrpol (Gene ID:5442; UniProt:O00411)
    
    * For research use only
- Applications
  - Application
    
    ELISA
    
    Application Description
    
    ELISA-antibody detection limit dilution 1:128000.
    WB-Preliminary experiments gave bands at approx 90kDa and 35kDa in Human HepG2 lysates after 1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 139kDa according to NP_005026.3. Both detected bands were successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field-have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

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