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Overview
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This gene encodes the largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The product of this gene contains a carboxy terminal domain composed of heptapeptide repeats that are essential for polymerase activity. These repeats contain serine and threonine residues that are phosphorylated in actively transcribing RNA polymerase. In addition, this subunit, in combination with several other polymerase subunits, forms the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA.
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- Properties
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Overview
Immunoprecipitation analysis of 200ug extracts of HeLa cells using 3ug POLR2A antibody (STJ113726). Western blot was performed from the immunoprecipitate using POLR2A antibody (STJ113726) at a dilition of 1:1000.
Immunohistochemistry of paraffin-embedded rat testis using POLR2A antibody (STJ113726) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse testis using POLR2A antibody (STJ113726) at dilution of 1:100 (40x lens).
Western blot analysis of extracts of various cell lines, using POLR2A antibody (STJ113726) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 30s.
Anti-POLR2A antibody
Anti-POLR2A antibody
Catalog Number:
A001020860STJ
Mfr. No.:
STJ113726
Price:
$297
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