Name: Anti-Phospho-YAP (S127) antibody
Price: 224.00 USD

Overview
- YAP is a protein encoded by the YAP1 gene which is approximately 54, 5 kDa. YAP is localised to the cytoplasm and nucleus. It is involved in signalling by ERBB4, gene expression, metabolism and hippo signalling pathway. It is a transcriptional regulator which can act both as a coactivator and a corepressor and is the critical downstream regulatory target in the Hippo signalling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. YAP is expressed in the liver, lung, nervous system, pancreas and skin with increased expression seen in some liver and prostate cancers. Mutations in the YAP1 gene may result in Coloboma.This primary antibody specifically binds to endogenous YAP protein which only binds about S127 when S127 is phosphorylated.
  
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Properties
- Host
  
  rabbit
  
  Antibody Type
  
  polyclonal
  
  Isotype
  
  IgG
  
  Reactivity
  
  human; mouse; rat; monkey
  
  Immunogen
  
  Synthesized peptide derived from human YAP around the phosphorylation site of S127.
  
  Purification
  
  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
  
  Conjugate
  
  Unconjugated
  
  Formulation
  
  Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
  
  Storage
  
  Store at -20°C and avoid repeat freeze-thaw cycles.
  
  Concentration
  
  1 mg/ml
  
  Regulatory Status
  
  RUO
  
  Antigen
  
  Transcriptional Coactivator Yap1; Yes-Associated Protein 1; Protein Yorkie Homolog; Yes-Associated Protein Yap65 Homolog (Gene ID:10413; UniProt:P46937)
  
  * For research use only

Applications

Application

IF; WB; IHC (paraffin); ELISA

Application	Dilutions
IF	1:50-200
WB	1:500-1:2000
IHC	1:100-1:300
ELISA	1:40000

Immunohistochemistry analysis of paraffin-embedded human heart, using YAP (Phospho-Ser127) Antibody. The picture on the right is blocked with the phospho peptide.

Western blot analysis of lysates from COS7 cells treated with HU 2nM 24h, using YAP (Phospho-Ser127) Antibody. The lane on the right is blocked with the phospho peptide.

Western blot analysis of MCF7 cells using Phospho-YAP (S127) Polyclonal Antibody diluted at 1：500 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Western blot analysis of various cells using Phospho-YAP (S127) Polyclonal Antibody diluted at 1：500 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-Phospho-YAP (S127) antibody

Anti-Phospho-YAP (S127) antibody

Immunohistochemistry analysis of paraffin-embedded human heart, using YAP (Phospho-Ser127) Antibody. The picture on the right is blocked with the phospho peptide.

Western blot analysis of lysates from COS7 cells treated with HU 2nM 24h, using YAP (Phospho-Ser127) Antibody. The lane on the right is blocked with the phospho peptide.

Western blot analysis of MCF7 cells using Phospho-YAP (S127) Polyclonal Antibody diluted at 1：500 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Western blot analysis of various cells using Phospho-YAP (S127) Polyclonal Antibody diluted at 1：500 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-Phospho-YAP (S127) antibody

Anti-Phospho-YAP (S127) antibody

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