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Overview
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This gene is highly similar to the v-src gene of Rous sarcoma virus. This proto-oncogene may play a role in the regulation of embryonic development and cell growth. The protein encoded by this gene is a tyrosine-protein kinase whose activity can be inhibited by phosphorylation by c-SRC kinase. Mutations in this gene could be involved in the malignant progression of colon cancer. Two transcript variants encoding the same protein have been found for this gene.
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Overview
Western blot analysis of extracts of NIH/3T3 cells, using Phospho-SRC Family-Y416 pAb (STJ11100962) at 1:1000 dilution or SRC antibody ( A18240).NIH/3T3 cells were treated by PDGF (100 ng/mL) at 37°C for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit.Exposure time: 60s.
Western blot analysis of extracts of NIH/3T3-Src cells, using Phospho-SRC Family-Y416 antibody (STJ11100962).Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.
Anti-Phospho-SRC Family-Y416 antibody
Anti-Phospho-SRC Family-Y416 antibody
Catalog Number:
A001018653STJ
Mfr. No.:
STJ11100962
Price:
$295
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