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Western blot analysis of extracts of HeLa cells, using Phospho-EIF4B-S422 antibody (STJ116395) at 1:2000 dilution. HeLa cells were treated by EGF (100ng/mL) for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit.Exposure time: 60s.

Western blot analysis of extracts of HeLa cells, using Phospho-EIF4B-S422 antibody (STJ116395) at 1:2000 dilution. HeLa cells were treated by EGF (100ng/mL) for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit.Exposure time: 60s.

Anti-Phospho-EIF4B-(S422) antibody

Anti-Phospho-EIF4B-(S422) antibody

Catalog Number:

A001023252STJ

Mfr. No.:

STJ116395

Price:

$295

Size:

Quantity:

Add to Cart:

Overview
- Please contact us at for specific academic pricing.
Properties
- Host
  
  rabbit
  
  Antibody Type
  
  polyclonal
  
  Isotype
  
  IgG
  
  Reactivity
  
  human
  
  Immunogen
  
  A synthetic phosphorylated peptide around S422 of human EIF4B (NP_001408. 2).
  
  Purification
  
  Affinity purification
  
  Conjugate
  
  Unconjugated
  
  Formulation
  
  PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
  
  Storage
  
  Store at -20°C and avoid freeze-thaw cycles.
  
  Regulatory Status
  
  RUO
  
  Antigen
  
  Eukaryotic Translation Initiation Factor 4b; Eif-4b (Gene ID:1975; UniProt:P23588)
  
  * For research use only

Applications

Application

WB

Application	Dilutions
WB	1:500-1:2000

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