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Overview
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This gene encodes a member of the serine/threonine protein kinase family. The encoded protein, a component of a cytokine-activated protein complex that is an inhibitor of the essential transcription factor NF-kappa-B complex, phosphorylates sites that trigger the degradation of the inhibitor via the ubiquination pathway, thereby activating the transcription factor.
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- Properties
- Applications
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Overview
Western blot analysis of extracts of HeLa cells, using Phospho-IKKα-S176 pAb (STJ117872) at 1:1000 dilution or IKK alpha antibody (STJ116403).HeLa cells were treated by TNF-α (20 ng/mL) at 37°C for 30 minutes.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit.Exposure time: 30s.
Western blot analysis of extracts of C6 cells, using Phospho-IKKα-S176 antibody (STJ117872) at 1:1000 dilution.C6 cells were treated by TNF-α (20 ng/mL) at 37°C for 30 minutes.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit.Exposure time: 30s.
Anti-Phospho-CHUK-(S176) antibody
Anti-Phospho-CHUK-(S176) antibody
Catalog Number:
A001024713STJ
Mfr. No.:
STJ117872
Price:
$295
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