Anti-Phospho-ACTB-Tyr-53 antibody

Anti-Phospho-ACTB-Tyr-53 antibody

Catalog Number:
A001480541STJ
Mfr. No.:
STJA0005242
Price:
$596
  • Size:
    100 µL
    Quantity:
    Add to Cart:
      • Overview
        • This antibody detects a 42 kDa* protein corresponding to the molecular mass of Actin on SDS-PAGE immunoblots of pervanadate treated human C2C12 and SYF cSrc transformed cells, but not in control cells. In addition, this antibody either detects actin cleavage products at 36 and 20 kDa, or cross-reacts with unidentified proteins at these molecular weights in C2C12 cells.

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      • Properties
        • Host
          rabbit
          Antibody Type
          polyclonal
          Isotype
          IgG
          Reactivity
          chicken; human; mouse; rat
          Immunogen
          Phospho-Actin (Tyr-53) synthetic peptide (coupled to KLH) corresponding to amino acid residues around tyrosine 53 of human Beta actin. This sequence is identical to similar regions in all four Alpha actins, as well as in Gamma actin, and is well conserved in actins from most eukaryotic species.
          Purification
          Antigen Affinity Purified
          Conjugate
          Unconjugated
          Formulation
          PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
          Storage
          Store at -20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
          Antigen
          (UniProt:P60709)

          * For Research Use Only (RUO). Not Used in Diagnostic or Therapeutic Applications.

      • Applications
        • Application
          WB; ICC
        • Application Dilutions
          WB 1:1000
          ICC 1:50

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