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Overview
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Clone M008 detects a 42 kDa* protein corresponding to the molecular mass of Beta-actin on SDS-PAGE immunoblots of human cancer cell lines, as well as human recombinant Beta-actin. This actin antibody preferentially detects human Beta-actin with only weak reactivity toward actins in rat, mouse, or rabbit. The antibody works in multiple applications including western blot, immunocytochemical labeling, ELISA, and immunoprecipitation. In addition, mass spectrometry analysis of immunoprecipitates using AM0081 in human A431 cell lysate confirmed that this antibody detects Beta-actin.
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