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Overview
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PDK1 is a protein encoded by the PDPK1 gene which is approximately 63, 1 kDa. PDK1 is localised to the cytoplasm, nucleus and cell membrane. It is involved in RET signalling, IL-2 pathway, regulation of lipid metabolism and development HGF signalling pathway. It is a serine/threonine kinase which acts as a master kinase, phosphorylating and activating a subgroup of the AGC family of protein kinases. It also plays a central role in the transduction of signals from insulin by providing the activating phosphorylation to PKB/AKT1, therefore propagating the signal to downstream targets controlling cell proliferation and survival, as well as glucose and amino acid uptake and storage. PDK1 is expressed ubiquitously in human tissues. STJ95010 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. This polyclonal antibody detects endogenous levels of PDK1 protein.
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Overview
Western blot analysis of lysates from MDA-MB-435 cells, treated with EGF, using PDK1 Antibody. The lane on the right is blocked with the synthesized peptide.
Western blot analysis of lysates from 1) Hela, 2) COLO, 3) 293T cells, （Green） primary antibody was diluted at 1:1000, 4°C over night, secondary antibody (cat: (NA) was diluted at 1:10000, 37°C 1hour. （Red） GAPDH monoclonal antibody (2B8) (cat: (STJ96931) antibody was diluted at 1:5000 as loading control, 4°C over night, secondary antibody (cat: (NA) was diluted at 1:10000, 37°C 1hour.
Western blot analysis of COLO205 cells using PDK1 Polyclonal Antibody diluted at 1：1000 Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using PDK1 Antibody. The picture on the right is blocked with the synthesized peptide.
Immunofluorescence analysis of rat-spleen tissue. 1, PDK1 Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B Western blot analysis of various cells using PDK1 Polyclonal Antibody diluted at 1：1000 Immunofluorescence analysis of rat-spleen tissue. 1, PDK1 Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B Immunofluorescence analysis of rat-lung tissue. 1, PDK1 Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B Immunofluorescence analysis of rat-lung tissue. 1, PDK1 Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B Immunofluorescence analysis of rat-heart tissue. 1, PDK1 Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B Immunofluorescence analysis of rat-heart tissue. 1, PDK1 Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Anti-PDK1 antibody
Anti-PDK1 antibody
Catalog Number:
A001050380STJ
Mfr. No.:
STJ95010
Price:
$224
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