Name: Anti-PDE4D antibody
Price: 499.00 USD

- Overview
  - Please contact us at for specific academic pricing.
- Properties
  - Host
    
    goat
    
    Antibody Type
    
    polyclonal
    
    Reactivity
    
    human
    
    Purification
    
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    
    Conjugate
    
    Unconjugated
    
    Formulation
    
    Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
    
    Storage
    
    Store at -20°C and avoid freeze-thaw cycles.
    
    Concentration
    
    0.5 mg/ml
    
    Regulatory Status
    
    RUO
    
    Antigen
    
    Camp-Specific 3'-5'-Cyclic Phosphodiesterase 4d; Dpde3; Pde43 (Gene ID:5144; UniProt:Q08499)
    
    * For research use only
- Applications
  - Application
    
    ELISA; WB; IHC; EIA
    
    Application Description
    
    ELISA-antibody detection limit dilution 1:128000.
    WB-Approx 70+80+90kDa bands observed in lysates of cell line A549. In transfected COS1 transiently expressing PDE4D1 and PDE4D3 bands of approx. 68kDa and 95kDa are observed. These bands are not observed in the untransfected COS1. Calculated MW of 91.1kDa according to PDE4D1, 76.5kDa according to PDE4D2 and 84.7kDa according to PDE4D3. Recommended concentration: 0.1-0.3µg/ml.
    IHC-Paraffin embedded Human Skeletal Muscle and Heart. Recommended concentration: 5µg/ml.
    IF-ELISA: This antibody has been successfully used in ELISA as described in the following paper: Bazhin et al, Mol Immunol. 2010 Jan;47:678-84.; PMID: 19939455.