STJ72523 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane and nuclear staining. The nuclear stain i STJ72523 Immunofluorescence analysis of paraformaldehyde fixed NIH3T3 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. The nuclear stain is DAPI (bl

Anti-NOTCH4 antibody

Catalog Number:

A001044291STJ

Mfr. No.:

STJ72523

Price:

$499

Size:

100 µg

Quantity:

Add to Cart:

Add to Cart

- Overview
  - Please contact us at for specific academic pricing.
- Properties
  - Host
    
    goat
    
    Antibody Type
    
    polyclonal
    
    Reactivity
    
    human; mouse; rat; dog; pig; cow
    
    Purification
    
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    
    Conjugate
    
    Unconjugated
    
    Formulation
    
    Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
    
    Storage
    
    Store at -20°C and avoid freeze-thaw cycles.
    
    Concentration
    
    0.5 mg/ml
    
    Regulatory Status
    
    RUO
    
    Antigen
    
    Neurogenic Locus Notch Homolog Protein 4; Notch 4; Hnotch4 Cleaved Into-Notch 4 Extracellular Truncation-Notch 4 Intracellular Domain (Gene ID:4855; UniProt:Q99466)
    
    * For research use only
- Applications
  - Application
    
    ELISA; IF
    
    Application Description
    
    ELISA-antibody detection limit dilution 1:8000.
    WB-Preliminary experiments gave an approx 27kDa band in all human, mouse and rat tissue and cell line lysates tested after 1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 210kDa according to NP_004548.3. The 27kDa band was successfully blocked by incubation with the immunizing peptide.
    IF-Strong expression of the protein seen in the nuclei and membranes of HeLa cells, and in the membranes of NIH3T3 cells. Recommended concentration: 10µg/ml.

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