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Overview
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NOS3 is a protein encoded by the NOS3 gene which is approximately 133, 2 kDa. NOS3 is localised to the cell membrane, Golgi apparatus and cytoplasm. It is involved in eNOS activation and regulation, activation of cAMP-dependent PKA, arginine biosynthesis and vascular smooth muscle contraction. It is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. Together with inducible nitric oxide synthase and neuronal nitric oxide synthase, it catalyses nitric oxide and L-citrulline generation from L-arginine and molecular oxygen. NOS3 is expressed in platelets, placenta, liver and kidney. Mutations in the NOS3 gene may result in Alzheimer disease and hypertension. STJ94539 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. This polyclonal antibody detects endogenous levels of NOS3 protein.
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Overview
Western blot analysis of mouse-brain lysis using NOS3 antibody. Antibody was diluted at 1:1000 Western blot analysis of A549 cells using NOS3 Polyclonal Antibody diluted at 1：1000 Western blot analysis of A549 cells using NOS3 Polyclonal Antibody diluted at 1：1000 Immunohistochemical analysis of paraffin-embedded Rat-spleen tissue. 1, NOS3 Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Mouse-testis tissue. 1, NOS3 Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
Western blot analysis of lysates from A549, K562, and COLO205 cells, using eNOS Antibody. The lane on the right is blocked with the synthesized peptide.
Immunofluorescence analysis of rat-lung tissue. 1, NOS3 Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B Immunohistochemical analysis of paraffin-embedded Human-uterus-cancer tissue. 1, NOS3 Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Human-stomach tissue. 1, NOS3 Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunofluorescence analysis of rat-spleen tissue. 1, NOS3 Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B Immunofluorescence analysis of rat-spleen tissue. 1, NOS3 Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B Immunofluorescence analysis of rat-kidney tissue. 1, NOS3 Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B Immunofluorescence analysis of rat-kidney tissue. 1, NOS3 Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B Immunofluorescence analysis of rat-lung tissue. 1, NOS3 Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B Western blot analysis of mouse-brain lysis using NOS3 antibody. Antibody was diluted at 1:1000 Immunohistochemical analysis of paraffin-embedded Rat-testis tissue. 1, NOS3 Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunofluorescence analysis of HeLa cells, using eNOS Antibody. The picture on the right is blocked with the synthesized peptide.
Immunohistochemical analysis of paraffin-embedded Rat-brain tissue. 1, NOS3 Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
Western blot analysis of the lysates from HepG2 cells using eNOS antibody.
Immunofluorescence analysis of Hela cell. 1, NOS3 Polyclonal Antibody (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). 3 DAPI (blue) 10min.
Anti-NOS3 antibody
Anti-NOS3 antibody
Catalog Number:
A001049909STJ
Mfr. No.:
STJ94539
Price:
$224
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