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Overview
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This gene encodes a member of the NimA (never in mitosis A) family of serine/threonine protein kinases. The encoded protein differs from other NimA family members in that it is not cell cycle regulated and is found primarily in the cytoplasm. The kinase is activated by prolactin stimulation, leading to phosphorylation of VAV2 guanine nucleotide exchange factor, paxillin, and activation of the RAC1 GTPase. Multiple transcript variants encoding different isoforms have been found for this gene.
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- Properties
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Overview
Immunofluorescence analysis of HeLa cells using NEK3 antibody (STJ28748). Blue: DAPI for nuclear staining.
Western blot analysis of extracts of various cell lines, using NEK3 antibody (STJ28748) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 1s.
Anti-NEK3 antibody
Anti-NEK3 antibody
Catalog Number:
A001036819STJ
Mfr. No.:
STJ28748
Price:
$297
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