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Overview
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This gene encodes a member of the neuronal calcium sensor (NCS) family of calcium-binding proteins. The protein contains an N-terminal myristoylation signal and four EF-hand calcium binding loops. The protein is cytosolic at resting calcium levels; however, elevated intracellular calcium levels induce a conformational change that exposes the myristoyl group, resulting in protein association with membranes and partial co-localization with the perinuclear trans-golgi network. The protein is thought to be a regulator of G protein-coupled receptor signal transduction. Several alternatively spliced variants of this gene have been determined, all of which encode the same protein; additional variants may exist but their biological validity has not been determined.
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- Properties
- Applications
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Overview
Immunofluorescence analysis of A549 cells using NCALD antibody (STJ110307). Blue: DAPI for nuclear staining.
Western blot analysis of extracts of various cell lines, using NCALD antibody (STJ110307) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.
Anti-NCALD antibody
Anti-NCALD antibody
Catalog Number:
A001017152STJ
Mfr. No.:
STJ110307
Price:
$297
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