Name: Anti-NANOG antibody
Price: 499.00 USD

- Overview
  - Please contact us at for specific academic pricing.
- Properties
  - Host
    
    goat
    
    Antibody Type
    
    polyclonal
    
    Reactivity
    
    human; dog; pig
    
    Purification
    
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    
    Conjugate
    
    Unconjugated
    
    Formulation
    
    Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
    
    Storage
    
    Store at -20°C and avoid freeze-thaw cycles.
    
    Concentration
    
    0.5 mg/ml
    
    Regulatory Status
    
    RUO
    
    Antigen
    
    Homeobox Protein Nanog; Homeobox Transcription Factor Nanog; Hnanog (Gene ID:79923; UniProt:Q9H9S0)
    
    * For research use only
- Applications
  - Application
    
    ELISA; WB; IF; IHC; EIA
    
    Application Description
    
    ELISA-antibody detection limit dilution 1:64000.
    WB-Approx 38kDa band observed in Human Ovary lysates. Recommended concentration: 0.03-0.1µg/ml. Primary incubation was 1 hour.
    IHC-This antibody was successfully used in: Vassena et al, Hum Mol Genet. 2012 Aug 1;21:3366-73. PMID: 22547223, Sancho-Martinez et al, Nat Commun. 2016 Feb 22;7:10743. PMID: 26899176, and Montserrat et al, Cell Transplant. 2012;21:815-25. PMID: 21944493.
    IF-IF/ICC: Parts of a keratinocyte-derived colony of induced pluripotent stem cells are stained for Nanog.
    This antibody was successfully used in IF on Human: PMID: 30878013, 21681858, 22613719, 21877920. 21285354, 19890879, 19483674 and 18931654. This antibody was successfully used in ICC on Human: PMID: 31479876, 29034898 and 29034891. Enzyme immunoassay: This antibody is reported to work in sandwich-type ELISA both as a capturer and as a reporter in combination with EB07693. This antibody does not work in PFA-fixed frozen embryos and ESC from Mouse.