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Overview
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Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein.
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Overview
Immunohistochemistry of paraffin-embedded Human colon using MRPL54 Rabbit pAb (STJ117156) at dilution of 1:100 (40x lens).
Western blot analysis of extracts of various cell lines, using MRPL54 antibody (STJ117156) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.
Anti-MRPL54 antibody
Anti-MRPL54 antibody
Catalog Number:
A001024005STJ
Mfr. No.:
STJ117156
Price:
$297
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