Name: Anti-Mouse MHC Class II In Vivo Antibody - Ultra Low Endotoxin (M5/114.15.2)
Price: 832.00 USD

- Overview
  - Antigen Distribution: I-Ab/d/q and I-Ed/k are expressed on antigen-presenting cells, including macrophages, monocytes, DCs, and B cells, and activated T cells from mice of the I-Ab/d/q and I-Ed/k haplotypes.
    Specificity: Clone M5/114.15.2 recognizes an epitope on the mouse MHC class II molecules I-Ab, I-Ad, I-Aq, I-Ed, and I-Ek. Clone M5/114.15.2 does not react with I-Af, I-Ak, or I-As.
    Purification method: This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
    Isotype Control: Rat IgG2b In Vivo Isotype Control - Low Endotoxin [1-2] (ICH2243)
    Endotoxin: ≤ 0.75 EU/mg as determined by the LAL method
    Aggregation: Aggregation level ≤ 1%
    
    Please contact us at for specific academic pricing.
    
    Background
    
    MHC class II antibody, clone M5/114.15.2, recognizes the major histocompatibility complex (MHC) class II molecules I-Ab, I-Ad, I-Aq, I-Ed, and I-Ek. MHC class II is constitutively expressed on professional antigen-presenting cells (APCs), including macrophages/monocytes, dendritic cells (DCs), and B cells, and is induced on T cells upon activation. MHC class II consists of two transmembrane proteins, a 35 kDa α (heavy) chain and 29 kDa β (light) chain. The N-terminal α1 and β1 domains form the antigen-binding groove, which binds 13-25 aa peptides derived from exogenous antigens. On APCs, MHC class II plays a critical role in the adaptive immune response by presenting phagocytosed antigens to helper CD4 T cells. The T cell receptor (TCR)/CD3 complex of CD4 T cells interacts with peptide-MHC class II, which induces CD4 T cell activation leading to the coordination and regulation of other effector cells. CD4 molecules also bind to MHC class II, which helps augment TCR signaling. It has also been demonstrated that MHC class II express on activated T cells are capable of antigen presentation and can transduce signals into T cells, enhancing T cell proliferation and activity.
- Properties
  - Host
    
    rat
    
    Isotype
    
    IgG2b
    
    Reactivity
    
    mouse
    
    Clone
    
    M5/114.15.2
    
    Immunogen
    
    C57BL/6 Spleen Cells
    
    Purification
    
    >98% by SDS-PAGE and HPLC
    
    Formulation
    
    This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.
    
    Storage
    
    This antibody is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.
    
    Molecular Weight
    
    ≥ 5.0 mg/ml
    
    Antigen
    
    (Gene ID:I-A/I-E MHC class II)
    
    * Products are for research use only. Not for use in diagnostic or therapeutic procedures.
- Applications
  - Application
    
    Blocking; CODEX®; Flow Cytometry; IHC (frozen); Immunoprecipitation

Anti-Mouse MHC Class II In Vivo Antibody - Ultra Low Endotoxin (M5/114.15.2)

Anti-Mouse MHC Class II In Vivo Antibody - Ultra Low Endotoxin (M5/114.15.2)

Background

We Also Recommend

Anti-Mouse CD22 In Vivo Antibody – Ultra Low Endotoxin (Cy34.1)

Anti-Mouse Fas Ligand In Vivo Antibody - Ultra Low Endotoxin (MFL3)

Bulk anti-Human CD1d antibody -Ultra Low Endotoxin (D5)