Name: Anti-Mouse Fas Ligand In Vivo Antibody - Ultra Low Endotoxin (MFL3)
Price: 832.00 USD

- Overview
  - Antigen Distribution: FasL is expressed on activated T cells, NK cells, the eye, and testis
    Specificity: Clone MFL3 recognizes an epitope on mouse FasL
    Purification method: This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
    Isotype Control: Armenian Hamster IgG Isotype Control for In Vivo - Ultra Low Endotoxin [PIP] (ICH2251UL)
    Endotoxin: ≤ 0.75 EU/mg as determined by the LAL method
    Aggregation: Aggregation level ≤ 1%
    
    Please contact us at for specific academic pricing.
    
    Background
    
    FasL antibody, clone AFS98, recognizes Fas ligand (FasL), also known as CD178, Apo-1 ligand, and CD95 ligand. FasL is a 40 kDa type II integral membrane protein that belongs to the tumor necrosis factor (TNF) superfamily. FasL is expressed by activated T cells and natural killer (NK cells). Binding of FasL to its receptor Fas (CD95, APO-1) induces apoptotic cell death in Fas-expressing target cells, contributing to anti-viral immunity. FasL also contributes to peripheral tolerance and the downregulation of immune responses through activation-induced autocrine and paracrine T cell death. FasL is also found in the anterior chamber of the eye and on Sertoli cells in the testis, and is implicated in immune-privilege at these sites. FasL also contributes to CD8 proliferation and neutrophil recruitment. Soluble FasL (26 kDa) can be released following cleavage by metalloproteinases and block FasL-mediated signaling. Fas/FasL-signaling is involved in the development of many human diseases, including autoimmunity and cancer. Many human tumors over-express FasL, resulting in tumor infiltrating lymphocyte (TIL) apoptosis and immune evasion, which is associated with poor prognosis.
- Properties
  - Host
    
    armenian hamster
    
    Isotype
    
    IgG
    
    Reactivity
    
    mouse
    
    Clone
    
    MFL3
    
    Immunogen
    
    B6 mouse FasL cDNA-transfected baby hamster kidney (B6 FasL/BHK) cells
    
    Purification
    
    >98% by SDS-PAGE and HPLC
    
    Formulation
    
    This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.
    
    Storage
    
    This antibody is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.
    
    Molecular Weight
    
    ≥ 5.0 mg/ml
    
    Regulatory Status
    
    The suggested concentration for this CD178 antibody, clone MFL3, for staining cells in flow cytometry is ≤ 0.25 μg per 10^6 cells in a volume of 100 μl. Titration of the reagent is recommended for optimal performance for each application.
    
    Antigen
    
    (Gene ID:FasL, Apo-1 Ligand, CD95 Ligand, TNFSF6, CD178)
    
    * Products are for research use only. Not for use in diagnostic or therapeutic procedures.
- Applications
  - Application
    
    Blocking; Flow Cytometry; Immunofluorescence

Anti-Mouse Fas Ligand In Vivo Antibody - Ultra Low Endotoxin (MFL3)

Anti-Mouse Fas Ligand In Vivo Antibody - Ultra Low Endotoxin (MFL3)

Background

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