Anti-Mouse CD8 beta In Vivo Antibody - Low Endotoxin (53-5.8)

Anti-Mouse CD8 beta In Vivo Antibody - Low Endotoxin (53-5.8)

Catalog Number:
A001437229ICH
Mfr. No.:
ICH1046
Price:
$680
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      • Overview
        • Antigen Distribution: Majority of thymocytes, cytotoxic T cell subsets
          Specificity: Anti-CD8 beta In Vivo Antibody - Low Endotoxin (53-5.8) recognizes an epitope on mouse CD8b.2 and antibody clone 53-5.8 is specific for Ly-3.2 and has low reactivity with Ly-3.1
          Purification method: This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
          Isotype Control: Rat IgG1 Isotype Control for In Vivo - Low Endotoxin [GL113] (ICH2246)
          Endotoxin: ≤ 1.0 EU/mg as determined by the LAL method
          Aggregation: Aggregation level ≤ 5%

          Please contact us at for specific academic pricing.

          Background

          CD8b is also known as Lyt-3.2 or Ly-3.2. It is a member of the Ig superfamily expressed as a heterodimer with the CD8A chain on a subset of MHC class I-restricted T cells and most thymocytes.

      • Properties
        • Host
          rat
          Isotype
          IgG1
          Reactivity
          mouse
          Clone
          53-5.8
          Immunogen
          Mouse thymus or spleen
          Purification
          >95% by SDS-PAGE and HPLC
          Formulation
          0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added.
          Storage
          anti-CD8 beta In Vivo Antibody - Low Endotoxin (53-5.8) is stable for at least one month when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.
          Molecular Weight
          1.0 - 5.0 mg/ml
          Regulatory Status
          Each investigator should determine their own optimal working dilution for specific applications.

          * Products are for research use only. Not for use in diagnostic or therapeutic procedures.

      • Applications
        • Application
          WB; Functional Assays; Flow Cytometry; Immunocytochemistry; IHC (frozen); Immunoprecipitation

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