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Anti-MHC II antibody [IBL-5/22]

Anti-MHC II antibody [IBL-5/22]

Catalog Number:

A001477907STJ

Mfr. No.:

STJ16102637

Price:

$451

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Quantity:

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Overview
- Please contact us at for specific academic pricing.
Properties
- Host
  
  rat
  
  Antibody Type
  
  monoclonal
  
  Isotype
  
  IgG kappa
  
  Reactivity
  
  mouse
  
  Clone
  
  IBL-5/22
  
  Immunogen
  
  A Wistar rat was immunized with mouse lymph node cells. Fusion partner: Sp2/0-Ag14.
  
  Purification
  
  The antibody is purified from tissue culture and contains no host Ig. Affinity purified from tissue culture.
  
  Conjugate
  
  Unconjugated
  
  Formulation
  
  PBS with 0.02% Sodium Azide.
  
  Storage
  
  Store for up to 1 year at 2-8°C upon receipt.
  
  Concentration
  
  Can be provided as 100 µg/mL, 500 µg/mL or 1mg/mL.
  
  Antigen
  
  (UniProt:P18468)
  
  * For Research Use Only (RUO). Not Used in Diagnostic or Therapeutic Applications.

Applications

Application

FC; WB; IF; IHC; IP

Application	Dilutions
Flow cytometry	0.5-1.0 µg/million cells in 0.1 ml
Immunoblot	1-2 µg/ml
Immunofluorescence	0.5-1.0 µg/ml
Immunohistology	1-2 µg/ml for 30 min at RT, staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
Immunoprecipitation	1-2 µg per 100-500 µg cell lysate protein/1 ml of anti-mouse coated Sepharose-4B suspension.

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