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Anti-BrdU antibody [85-2C8]

Anti-BrdU antibody [85-2C8]

Catalog Number:
A001477910STJ
Mfr. No.:
STJ16102640
Price:
$451
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      • Overview

        • Please contact us at for specific academic pricing.

      • Properties
        • Host
          mouse
          Antibody Type
          monoclonal
          Isotype
          IgG1 kappa
          Reactivity
          all species
          Clone
          85-2C8
          Immunogen
          A BALB/c mouse was immunized with a conjugate of bromodeoxyrudine and Helix pomatia hemocyanin (HPH).The conjugate was prepared using periodate oxidation. Fusion partner: P3-X63-Ag8.653.
          Purification
          The antibody is purified from tissue culture and contains no host Ig. Affinity purified from tissue culture.
          Conjugate
          Unconjugated
          Formulation
          PBS with 0.02% Sodium Azide.
          Storage
          Store for up to 1 year at 2-8°C upon receipt.
          Concentration
          Can be provided as 100 µg/mL, 500 µg/mL or 1mg/mL.

          * For Research Use Only (RUO). Not Used in Diagnostic or Therapeutic Applications.

      • Applications
        • Application
          FC; IF; IHC
        • Application Dilutions
          Flow cytometry 0.5-1.0 µg/million cells in 0.1 ml, fix cells in 4% PFA for 10 min, at 4°C, permeabilize with 0, 2% saponin or digitonin for 15 min, at 4°C
          Immunofluorescence 0.5-1.0 µg/ml
          Immunohistology 2-4 µg/ml for 30 min at RT, staining of formalin-fixed tissues requires pre-treatment of sections with 4N HCl for 30 minutes at RT to denature double-stranded DNA, followed by digestion with trypsin at 1 mg/ml PBS for 20 min at RT or 10 min at 37°C.

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