STJ70015 (0.1µg/ml) staining of Human Testis lysate (RIPA buffer, 1.4E5 cells per lane). Detected by chemiluminescence.

Anti-MELK antibody

Name: Anti-MELK antibody
Price: 499.00 USD

Catalog Number:

A001041832STJ

Mfr. No.:

STJ70015

Price:

$499

Size:

100 µg

Quantity:

Add to Cart:

Add to Cart

- Overview
  - Please contact us at for specific academic pricing.
- Properties
  - Host
    
    goat
    
    Antibody Type
    
    polyclonal
    
    Reactivity
    
    human; mouse; dog; cow
    
    Purification
    
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    
    Conjugate
    
    Unconjugated
    
    Formulation
    
    Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
    
    Storage
    
    Store at -20°C and avoid freeze-thaw cycles.
    
    Concentration
    
    0.5 mg/ml
    
    Regulatory Status
    
    RUO
    
    Antigen
    
    Maternal Embryonic Leucine Zipper Kinase; Hmelk; Protein Kinase Eg3; Peg3 Kinase; Protein Kinase Pk38; Hpk38; Tyrosine-Protein Kinase Melk (Gene ID:9833; UniProt:Q14680)
    
    * For research use only
- Applications
  - Application
    
    ELISA; WB
    
    Application Description
    
    ELISA-antibody detection limit dilution 1:32000.
    WB-Experiments gave bands at approx 75kDa and 150kDa in Human Testis lysates and in cell lines HeLa and Jurkat after 0.1µg/ml antibody staining. The150kDa band is explained by glycosylation of this member of the AMPK family. This protein has a calculated MW of 74.6kDa according to NP_055606.1. Recommended concentration: 0.1-0.3µg/ml. Preliminary testing was unsuccessful on Mouse Testis for this particular batch.

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