STJ72310 Immunofluorescence analysis of paraformaldehyde fixed L929 cells (Mouse fibroblast cell line), permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. Th STJ72310 Immunofluorescence analysis of paraformaldehyde fixed NIH3T3 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. The nuclear stain is DAPI (bl STJ72310 Flow cytometric analysis of paraformaldehyde fixed NIH3T3 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) f

Anti-LY6E antibody

Catalog Number:

A001044082STJ

Mfr. No.:

STJ72310

Price:

$499

Size:

100 µg

Quantity:

Add to Cart:

Add to Cart

- Overview
  - Please contact us at for specific academic pricing.
- Properties
  - Host
    
    goat
    
    Antibody Type
    
    polyclonal
    
    Reactivity
    
    mouse
    
    Purification
    
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    
    Conjugate
    
    Unconjugated
    
    Formulation
    
    Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
    
    Storage
    
    Store at -20°C and avoid freeze-thaw cycles.
    
    Concentration
    
    0.5 mg/ml
    
    Regulatory Status
    
    RUO
    
    Antigen
    
    Lymphocyte Antigen 6e; Ly-6e; Retinoic Acid-Induced Gene E Protein; Rig-E; Stem Cell Antigen 2; Sca-2; Thymic Shared Antigen 1; Tsa-1 (Gene ID:4061; UniProt:Q16553)
    
    * For research use only
- Applications
  - Application
    
    ELISA; IF; Flow Cytometry; IHC
    
    Application Description
    
    ELISA-antibody detection limit dilution 1:16000.
    WB-Preliminary experiments in Mouse Brain, Kidney, Heart and Spleen lysates gave no specific signal but low background.
    IHC-Customer obtained staining in Mouse Placenta in a pattern consistent with in situ hybridisation results, and no staining in KO mice. This antibody has been successfully used in IHC on Mouse: M. Hughes et al, Placenta. 2013 Sep;34:831-5. PMID: 23830620.
    IF-Strong expression of the protein seen in the membranes of NIH3T3 and L929 cells. Recommended concentration: 10µg/ml.
    FC-Flow cytometric analysis of NIH3T3 cells. Recommended concentration: 10ug/ml.