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Anti-Keratin 8 antibody [E2]

Anti-Keratin 8 antibody [E2]

Catalog Number:

A001477916STJ

Mfr. No.:

STJ16102646

Price:

$451

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Quantity:

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Overview
- Please contact us at for specific academic pricing.
Properties
- Host
  
  mouse
  
  Antibody Type
  
  monoclonal
  
  Isotype
  
  IgG1 kappa
  
  Reactivity
  
  R.Temporaria; X.laevis; rat
  
  Clone
  
  E2
  
  Immunogen
  
  A BALB/c mouse was immunized with an intermediate filament preparation of rat colon mucosa.
  
  Purification
  
  The antibody is purified from tissue culture and contains no host Ig. Affinity purified from tissue culture.
  
  Conjugate
  
  Unconjugated
  
  Formulation
  
  PBS with 0.02% Sodium Azide.
  
  Storage
  
  Store for up to 1 year at 2-8°C upon receipt.
  
  Concentration
  
  Can be provided as 100 µg/mL, 500 µg/mL or 1mg/mL.
  
  Antigen
  
  (UniProt:Q5BJY9)
  
  * For Research Use Only (RUO). Not Used in Diagnostic or Therapeutic Applications.

Applications

Application

FC; WB; IF; IHC; IP

Application	Dilutions
Flow Cytometry	1-2 µg/million cells in 0.1 ml, fix cells in 4% PFA for 10 min, at 4°C, permeabilize with 0, 2% saponin or digitonin for 15 min, at 4°C
Immunoblotting	1-2 µg/ml
Immunofluorescence	1-2 µg/ml
Immunohistology	1-2 µg/ml for 30 min at RT, for staining of formalin-fixed tissues no antigen retrieval method is known to date
Immunoprecipitation	1-2 µg per 100-500 µg cell lysate protein/1ml of anti-mouse coated Sepharose-4B suspension.

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