Name: Anti-KCNN4/KCa3.1 antibody
Price: 499.00 USD

- Overview
  - Please contact us at for specific academic pricing.
- Properties
  - Host
    
    goat
    
    Antibody Type
    
    polyclonal
    
    Reactivity
    
    human; mouse; rat; dog
    
    Purification
    
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    
    Conjugate
    
    Unconjugated
    
    Formulation
    
    Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
    
    Storage
    
    Store at -20°C and avoid freeze-thaw cycles.
    
    Concentration
    
    0.5 mg/ml
    
    Regulatory Status
    
    RUO
    
    Antigen
    
    Solute Carrier Family 12 Member 2; Basolateral Na-K-Cl Symporter; Bumetanide-Sensitive Sodium-(Potassium-Chloride Cotransporter 2 (Gene ID:6558; UniProt:P55011)
    
    * For research use only
- Applications
  - Application
    
    ELISA; WB; IF; Flow Cytometry; IHC
    
    Application Description
    
    ELISA-antibody detection limit dilution 1:16000.
    WB-Approx 50kDa band observed in Human Cerebellum lysates and in lysates of cell line NIH3T3.An additional band of unknown identity was also consistently observed at 35kDa in Cerebellum. This band was successfully blocked by incubation with the immunising peptide. Recommended concentration: 0.1-0.3µg/ml. Primary incubation 1 hour at room temperature. Negative Control: Human Adrenal Gland lysate.
    IHC-Paraffin embedded Human Adrenal Gland, Kidney and Liver. Recommended concentration: 3.75µg/ml.
    IF-Strong expression of the protein seen in the cytoplasm/membranes of HeLa cells. Recommended concentration: 10µg/ml.
    FC-Flow cytometric analysis of HEK293 cells. Recommended concentration: 10ug/ml.