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Overview
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Jagged1 is a protein encoded by the JAG1 gene which is approximately 133, 7 kDa. Jagged1 is localised to the plasma membrane. It is involved in signalling by Notch1, Notch2 activation and transmission of signal to the nucleus and the HIV life cycle. It is a ligand for multiple Notch receptors and may also be involved in cell-fate decisions during hematopoiesis and in the early and late stages of mammalian cardiovascular development. Jagged1 is widely expressed in adult and foetal tissues. Mutations in the JAG1 gene may result in Alagille syndrome. STJ96766 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. This polyclonal antibody detects endogenous levels of Jagged1 protein.
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Overview
Western blot analysis of lysate from HeLa cells, using JAG1 Antibody.
Immunohistochemical analysis of paraffin-embedded mouse-kidney, antibody was diluted at 1:100 Immunohistochemical analysis of paraffin-embedded mouse-kidney, antibody was diluted at 1:100 Western blot analysis of HeLa cells using Jagged1 Polyclonal Antibody.. Secondary antibody was diluted at 1:20000 Immunohistochemical analysis of paraffin-embedded mouse-kidney, antibody was diluted at 1:100 Immunofluorescence analysis of rat-spleen tissue. 1, Jagged1 Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B Immunohistochemical analysis of paraffin-embedded Rat-spleen tissue. 1, Jagged1 Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunofluorescence analysis of rat-spleen tissue. 1, Jagged1 Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B Immunofluorescence analysis of human-stomach tissue. 1, Jagged1 Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B Immunofluorescence analysis of human-stomach tissue. 1, Jagged1 Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B Immunofluorescence analysis of human-liver tissue. 1, Jagged1 Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B Immunofluorescence analysis of human-liver tissue. 1, Jagged1 Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Anti-Jagged1 antibody
Anti-Jagged1 antibody
Catalog Number:
A001052136STJ
Mfr. No.:
STJ96766
Price:
$224
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