Name: Anti-ITM2B antibody
Price: 499.00 USD

- Overview
  - Please contact us at for specific academic pricing.
- Properties
  - Host
    
    goat
    
    Antibody Type
    
    polyclonal
    
    Reactivity
    
    human; mouse; rat; cow
    
    Purification
    
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    
    Conjugate
    
    Unconjugated
    
    Formulation
    
    Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
    
    Storage
    
    Store at -20°C and avoid freeze-thaw cycles.
    
    Concentration
    
    0.5 mg/ml
    
    Regulatory Status
    
    RUO
    
    Antigen
    
    Integral Membrane Protein 2b; Immature Bri2; Imbri2; Protein E25b; Transmembrane Protein Bri; Bri Cleaved Into-Bri2-Membrane Form; Mature Bri2; Mbri2-Bri2 Intracellular Domain; Bri2 Icd-Bri2c-Soluble Form-Bri23 Peptide; Bri2-23; Abri23; C-Terminal Peptide; P23 Peptide (Gene ID:9445; UniProt:Q9Y287)
    
    * For research use only
- Applications
  - Application
    
    ELISA; WB; IHC; IF; Flow Cytometry
    
    Application Description
    
    ELISA-antibody detection limit dilution 1:8000.
    WB-Approx. 30-35kDa band observed in HepG2 cell lysates. Recommended concentration: 0.1-0.3µg/ml. Primary incubation 1 hour at room temperature. Preliminary testing was unsuccessful on Rat and Mouse Brain for this particular batch.
    IHC-Paraffin embedded Human Small Intestine. Recommended concentration: 2.5µg/ml.
    IF-Strong expression of the protein seen in the cytoplasm and Golgi apparatus of A431 and HeLa cells. Recommended concentration: 10µg/ml.
    FC-Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.

STJ71822 (0.3µg/ml) staining of HepG2 cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Anti-ITM2B antibody

Anti-ITM2B antibody

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