Anti-ITCH/AIF4 antibody

Catalog Number:

A001042901STJ

Mfr. No.:

STJ71108

Price:

$499

Size:

100 µg

Quantity:

Add to Cart:

Add to Cart

- Overview
  - Please contact us at for specific academic pricing.
- Properties
  - Host
    
    goat
    
    Antibody Type
    
    polyclonal
    
    Reactivity
    
    human; mouse; rat; dog; cow
    
    Purification
    
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    
    Conjugate
    
    Unconjugated
    
    Formulation
    
    Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
    
    Storage
    
    Store at -20°C and avoid freeze-thaw cycles.
    
    Concentration
    
    0.5 mg/ml
    
    Regulatory Status
    
    RUO
    
    Antigen
    
    E3 Ubiquitin-Protein Ligase Itchy Homolog; Itch; Atrophin-1-Interacting Protein 4; Aip4; Hect-Type E3 Ubiquitin Transferase Itchy Homolog; Nfe2-Associated Polypeptide 1; Napp1 (Gene ID:83737; UniProt:Q96J02)
    
    * For research use only
- Applications
  - Application
    
    ELISA; WB; IF; Flow Cytometry
    
    Application Description
    
    ELISA-antibody detection limit dilution 1:16000.
    WB-Approx 90kDa band observed in Human Brain lysates. Recommended concentration: 0.1-0.3µg/ml. Primary incubation 1 hour at room temperature.
    IF-Strong expression of the protein seen in the nuclei of A431 and U2OS cells. Recommended concentration: 10µg/ml.
    FC-Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.