Name: Anti-HSPA5/GRP78 antibody
Price: 499.00 USD

- Overview
  - Please contact us at for specific academic pricing.
- Properties
  - Host
    
    goat
    
    Antibody Type
    
    polyclonal
    
    Reactivity
    
    human
    
    Purification
    
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    
    Conjugate
    
    Unconjugated
    
    Formulation
    
    Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
    
    Storage
    
    Store at -20°C and avoid freeze-thaw cycles.
    
    Concentration
    
    0.5 mg/ml
    
    Regulatory Status
    
    RUO
    
    Antigen
    
    Angiopoietin-1; Ang-1 (Gene ID:284; UniProt:Q15389)
    
    * For research use only
- Applications
  - Application
    
    ELISA; IF; WB
    
    Application Description
    
    ELISA-antibody detection limit dilution 1:128000.
    WB-Approx 75kDa band observed in lysates of cell lines HepG2, HeLa and MCF7, and in Mouse Liver and Testis lysates, and approx. 80kDa band in Rat Brain lysates. This molecular weight is routinely observed by other sources. Recommended concentration: 0.01-0.1µg/ml. Primary incubation was 1 hour.
    IF-Strong expression of the protein seen in the cytoplasm of HeLa cells. Recommended concentration: 5µg/ml.