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Overview
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In mammals, histamine is metabolized by two major pathways: N(tau)-methylation via histamine N-methyltransferase and oxidative deamination via diamine oxidase. This gene encodes the first enzyme which is found in the cytosol and uses S-adenosyl-L-methionine as the methyl donor. In the mammalian brain, the neurotransmitter activity of histamine is controlled by N(tau)-methylation as diamine oxidase is not found in the central nervous system. A common genetic polymorphism affects the activity levels of this gene product in red blood cells. Multiple alternatively spliced transcript variants that encode different proteins have been found for this gene.
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- Properties
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Overview
Immunofluorescence analysis of U2OS cells using HNMT antibody (STJ24061). Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 200ug extracts of HT-29 cells using 1ug HNMT antibody (STJ24061). Western blot was performed from the immunoprecipitate using HNMT antibody (STJ24061) at a dilition of 1:1000.____Detection: ECL Enhanced Kit._Exposure time: 10s.
Western blot analysis of extracts of various cell lines, using HNMT antibody (STJ24061) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.
Anti-HNMT antibody
Anti-HNMT antibody
Catalog Number:
A001034153STJ
Mfr. No.:
STJ24061
Price:
$297
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