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Overview
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Histone H2B is a protein encoded by the HIST1H2BA gene which is approximately 14, 1 kDa. Histone H2B is localised to the nucleus. It is involved in DNA double-strand break repair, cellular senescence, chromosome maintenance and mitotic cell cycle. Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units. Histone H2B is mainly expressed in testis, it is also found in some fat cells. STJ97064 was affinity-purified from rabbit antiserum by affinity-chromatography using specific immunogen. This antibody detects endogenous Histone H2B protein.
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Overview
Western blot analysis of 1) Hela, 2) 3T3, 3) Raw264.7, 4) Rat Brain, 5) Rat Kidney, diluted at 1:2000. cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).
Immunofluorescence analysis of Rat-heart tissue. 1, Histone H2B monoclonal antibody (Mix) (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B Immunofluorescence analysis of Human-liver-cancer tissue. 1, Histone H2B monoclonal antibody (Mix) (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B Immunofluorescence analysis of Mouse-kidney tissue. 1, Histone H2B monoclonal antibody (Mix) (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B Immunofluorescence analysis of Hela cell. 1, Brp44L Polyclonal Antibody (green) was diluted at 1:200 (4°C overnight). (red) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).
Anti-Histone H2B antibody
Anti-Histone H2B antibody
Catalog Number:
A001052433STJ
Mfr. No.:
STJ97064
Price:
$224
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