Name: Anti-Histone Deacetylase 1 antibody
Price: 499.00 USD

- Overview
  - Please contact us at for specific academic pricing.
- Properties
  - Host
    
    goat
    
    Antibody Type
    
    polyclonal
    
    Reactivity
    
    human; mouse; rat; dog
    
    Purification
    
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    
    Conjugate
    
    Unconjugated
    
    Formulation
    
    Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
    
    Storage
    
    Store at -20°C and avoid freeze-thaw cycles.
    
    Concentration
    
    0.5 mg/ml
    
    Regulatory Status
    
    RUO
    
    Antigen
    
    Histone Deacetylase 1; Hd1 (Gene ID:3065; UniProt:Q13547)
    
    * For research use only
- Applications
  - Application
    
    ELISA; WB; IF; ChIP
    
    Application Description
    
    ELISA-antibody detection limit dilution 1:4000.
    WB-Appox 55kDa band observed in Human Duodenum and Lymph Node lysates and approx 70kDa band observed in lysates of cell lines HeLa and NIH3T3. The observed 70kDa band represents the sumoylated protein according to David et al, J Biol Chem. 2002 Jun 28;277:23658-63; PMID: 11960997. Recommended concentration: 0.1-2µg/ml.
    IF-Strong expression of the protein seen in the nucleus of NIH3T3 cells. Recommended concentration: 10µg/ml Chromatin Immunoprecipitation: A 3.5-fold increased presence of H3 onto the Human P21 locus observed when comparing the precipitations using EB07186 with non-specific goat IgG to bring down HDAC1 in a chromatin lysate from MCF7 cells.