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Overview
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Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21. 3.
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Overview
Immunofluorescence analysis of U2OS cells using HIST3H3 antibody (STJ23980) at dilution of 1:100. Blue: DAPI for nuclear staining.
Chromatin immunoprecipitation analysis of extracts of HeLa cell line, using HIST3H3 antibody (STJ23980) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
Immunohistochemistry of paraffin-embedded mouse testis using Histone H3 antibody (STJ23980) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human appendix using Histone H3 antibody (STJ23980) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using Histone H3 antibody (STJ23980) at dilution of 1:100 (40x lens).
Western blot analysis of extracts of various cell lines, using HIST1H3A antibody (STJ23980) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 5s.
Western blot analysis of extracts of various cell lines, using HIST3H3 antibody (STJ23980) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.
Anti-HIST3H3 antibody
Anti-HIST3H3 antibody
Catalog Number:
A001034102STJ
Mfr. No.:
STJ23980
Price:
$297
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