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Overview
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The HIRA protein shares sequence similarity with Hir1p and Hir2p, the two corepressors of histone gene transcription characterized in the yeast, Saccharomyces cerevisiae. The structural features of the HIRA protein suggest that it may function as part of a multiprotein complex. Several cDNAs encoding HIRA-interacting proteins, or HIRIPs, have been identified. In vitro, the protein encoded by this gene binds HIRA, as well as H2B and H3 core histones, indicating that a complex containing HIRA-HIRIP3 could function in some aspects of chromatin and histone metabolism. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.
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Overview
Immunofluorescence analysis of U2OS cells using HIRIP3 antibody (STJ29094).
Immunohistochemistry of paraffin-embedded mouse liver using HIRIP3 antibody (STJ29094) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat spleen using HIRIP3 antibody (STJ29094) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human well-differentiated squamous skin carcinoma using HIRIP3 antibody (STJ29094) at dilution of 1:100 (40x lens).
Western blot analysis of extracts of various cell lines, using HIRIP3 antibody (STJ29094) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.
Anti-HIRIP3 antibody
Anti-HIRIP3 antibody
Catalog Number:
A001037122STJ
Mfr. No.:
STJ29094
Price:
$297
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