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Overview
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The human alpha globin gene cluster located on chromosome 16 spans about 30 kb and includes seven loci: 5'-zeta-pseudozeta-mu-pseudo alpha-1-alpha-2-alpha-1-theta-3'. The alpha-2 (HBA2) and alpha-1 (HBA1) coding sequences are identical. These genes differ slightly over the 5' untranslated regions and the introns, but they differ significantly over the 3' untranslated regions. Two alpha chains plus two beta chains constitute HbA, which in normal adult life comprises about 97% of the total hemoglobin; alpha chains combine with delta chains to constitute HbA-2, which with HbF (fetal hemoglobin) makes up the remaining 3% of adult hemoglobin. Alpha thalassemias result from deletions of each of the alpha genes as well as deletions of both HBA2 and HBA1; some nondeletion alpha thalassemias have also been reported.
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Overview
Immunofluorescence analysis of MCF7 cells using HBA1 antibody (STJ29461). Blue: DAPI for nuclear staining.
Western blot analysis of extracts of various cell lines, using HBA1 antibody (STJ29461) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit._Exposure time: 30s.
Anti-HBA1 antibody
Anti-HBA1 antibody
Catalog Number:
A001037445STJ
Mfr. No.:
STJ29461
Price:
$297
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