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Overview
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Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. The protein encoded by this gene is an enzyme belonging to the class II family of aminoacyl-tRNA synthetases. Functioning in the synthesis of histidyl-transfer RNA, the enzyme plays an accessory role in the regulation of protein biosynthesis. The gene is located in a head-to-head orientation with HARS on chromosome five, where the homologous genes likely share a bidirectional promoter. Mutations in this gene are associated with the pathogenesis of Perrault syndrome, which involves ovarian dysgenesis and sensorineural hearing loss. Alternative splicing results in multiple transcript variants of this gene.
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Overview
Immunohistochemistry of paraffin-embedded rat brain using HARS2 antibody (STJ110095) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse kidney using HARS2 antibody (STJ110095) at dilution of 1:100 (40x lens).
Western blot analysis of extracts of various cell lines, using HARS2 antibody (STJ110095) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 5s.
Anti-HARS2 antibody
Anti-HARS2 antibody
Catalog Number:
A001016955STJ
Mfr. No.:
STJ110095
Price:
$297
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