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Overview
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This gene encodes the alpha subunit of the mitochondrial trifunctional protein, which catalyzes the last three steps of mitochondrial beta-oxidation of long chain fatty acids. The mitochondrial membrane-bound heterocomplex is composed of four alpha and four beta subunits, with the alpha subunit catalyzing the 3-hydroxyacyl-CoA dehydrogenase and enoyl-CoA hydratase activities. Mutations in this gene result in trifunctional protein deficiency or LCHAD deficiency. The genes of the alpha and beta subunits of the mitochondrial trifunctional protein are located adjacent to each other in the human genome in a head-to-head orientation.
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- Properties
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Overview
Immunoprecipitation analysis of 200ug extracts of HeLa cells using 3ug HADHA antibody (STJ27299). Western blot was performed from the immunoprecipitate using HADHA antibody (STJ27299) at a dilition of 1:1000.
Western blot analysis of extracts from normal (control) and HADHA knockout (KO) 293T cells, using HADHA antibody (STJ27299) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 1s.
Immunofluorescence analysis of U2OS cells using HADHA antibody (STJ27299). Blue: DAPI for nuclear staining.
Western blot analysis of extracts of various cell lines, using HADHA antibody (STJ27299) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 3s.
Anti-HADHA antibody
Anti-HADHA antibody
Catalog Number:
A001035770STJ
Mfr. No.:
STJ27299
Price:
$295
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