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Overview
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The protein encoded by this gene binds the epidermal growth factor receptor and contains one SH2 domain and two SH3 domains. Its two SH3 domains direct complex formation with proline-rich regions of other proteins, and its SH2 domain binds tyrosine phosphorylated sequences. This gene is similar to the Sem5 gene of C. elegans, which is involved in the signal transduction pathway. Two alternatively spliced transcript variants encoding different isoforms have been found for this gene.
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- Properties
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Overview
Immunohistochemistry of paraffin-embedded mouse kidney using GRB2 antibody (STJ27656) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of HeLa cells using GRB2 antibody (STJ27656). Blue: DAPI for nuclear staining.
Western blot analysis of extracts of various cell lines, using GRB2 antibody (STJ27656) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.
Anti-GRB2 antibody
Anti-GRB2 antibody
Catalog Number:
A001036054STJ
Mfr. No.:
STJ27656
Price:
$297
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