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Overview
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In the de novo synthesis of purine nucleotides, IMP is the branch point metabolite at which point the pathway diverges to the synthesis of either guanine or adenine nucleotides. In the guanine nucleotide pathway, there are 2 enzymes involved in converting IMP to GMP, namely IMP dehydrogenase (IMPD1), which catalyzes the oxidation of IMP to XMP, and GMP synthetase, which catalyzes the amination of XMP to GMP.
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Overview
Immunoprecipitation analysis of 200ug extracts of MCF-7 cells, using 3 ug GMPS antibody (STJ28689). Western blot was performed from the immunoprecipitate using GMPS antibody (STJ28689) at a dilition of 1:1000.
Western blot analysis of extracts of various cell lines, using GMPS antibody (STJ28689) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.
Anti-GMPS antibody
Anti-GMPS antibody
Catalog Number:
A001036767STJ
Mfr. No.:
STJ28689
Price:
$297
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