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Overview
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Glut4 is a protein encoded by the SLC2A4 gene which is approximately 54, 7 kDa. Glut4 is localised to the cell membrane and cytoplasm. It is involved in the transport of glucose, other sugars, bile salts, organic acids and metal ions and AMP-activated protein kinase signalling. This protein falls under the solute carrier family 2 and functions as an insulin-regulated facilitative glucose transporter. In the absence of insulin, it is sequestered within the cells of muscle and adipose tissue. Within minutes of insulin stimulation, the protein moves to the cell surface and begins to transport glucose across the cell membrane. Glut4 is expressed in the skeletal and cardiac muscles, brown and white fat. Mutations in the SLC2A4 gene may be involved in diabetes mellitus. STJ96888 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. This polyclonal antibody detects endogenous levels of Glut4 protein.
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Overview
Immunohistochemical analysis of paraffin-embedded Mouse-kidney tissue. 1, Glut4 Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
Western blot analysis of SKOV3 cells using Glut4 Polyclonal Antibody.. Secondary antibody was diluted at 1:20000 Immunohistochemical analysis of paraffin-embedded Mouse-liver tissue. 1, Glut4 Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Mouse-heart tissue. 1, Glut4 Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Rat-spleen tissue. 1, Glut4 Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Rat-kidney tissue. 1, Glut4 Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Rat-lung tissue. 1, Glut4 Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
Anti-Glut4 antibody
Anti-Glut4 antibody
Catalog Number:
A001052258STJ
Mfr. No.:
STJ96888
Price:
$224
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