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Overview
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This gene encodes a member of the Kruppel family of zinc finger proteins. The encoded transcription factor is activated by the sonic hedgehog signal transduction cascade and regulates stem cell proliferation. The activity and nuclear localization of this protein is negatively regulated by p53 in an inhibitory loop. Multiple transcript variants encoding different isoforms have been found for this gene.
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Overview
Immunofluorescence analysis of NIH/3T3 cells using GLI1 antibody (STJ116877) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of HeLa cells using GLI1 antibody (STJ116877) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunohistochemistry of paraffin-embedded mouse testis using GLI1 antibody (STJ116877) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human mammary cancer using GLI1 antibody (STJ116877) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat testis using GLI1 antibody (STJ116877) at dilution of 1:100 (40x lens).
Western blot analysis of extracts of various cell lines, using GLI1 antibody (STJ116877) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.
Anti-GLI1 antibody
Anti-GLI1 antibody
Catalog Number:
A001023728STJ
Mfr. No.:
STJ116877
Price:
$297
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