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Overview
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This gene encodes one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
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- Properties
- Applications
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Overview
Immunofluorescence analysis of U-251MG cells using GFAP antibody (STJ23770) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunohistochemistry of paraffin-embedded rat brain using GFAP Rabbit pAb (STJ23770) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human brain using GFAP Rabbit pAb (STJ23770) at dilution of 1:100 (40x lens).
Western blot analysis of extracts of various cell lines, using GFAP Rabbit pAb (STJ23770) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 180s.
Anti-GFAP antibody
Anti-GFAP antibody
Catalog Number:
A001033988STJ
Mfr. No.:
STJ23770
Price:
$297
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