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Overview
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The hypoxia inducible factor (HIF) is a transcriptional complex that is involved in oxygen homeostasis. At normal oxygen levels, the alpha subunit of HIF is targeted for degration by prolyl hydroxylation. This gene encodes an enzyme responsible for this post-translational modification. Alternative splicing results in multiple transcript variants. Read-through transcription also exists between this gene and the upstream RAB4B (RAB4B, member RAS oncogene family) gene.
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Overview
Immunoprecipitation analysis of 150ug extracts of HeLa cells using 3ug EGLN2 antibody (STJ23493). Western blot was performed from the immunoprecipitate using EGLN2 antibody (STJ23493) at a dilition of 1:1000.
Western blot analysis of extracts of various cell lines, using EGLN2 antibody (STJ23493) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.
Anti-EGLN2 antibody
Anti-EGLN2 antibody
Catalog Number:
A001033824STJ
Mfr. No.:
STJ23493
Price:
$297
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