Name: Anti-EDF1/MBF1 antibody
Price: 499.00 USD

- Overview
  - Please contact us at for specific academic pricing.
- Properties
  - Host
    
    goat
    
    Antibody Type
    
    polyclonal
    
    Reactivity
    
    human; mouse; rat
    
    Purification
    
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    
    Conjugate
    
    Unconjugated
    
    Formulation
    
    Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
    
    Storage
    
    Store at -20°C and avoid freeze-thaw cycles.
    
    Concentration
    
    0.5 mg/ml
    
    Regulatory Status
    
    RUO
    
    Antigen
    
    Four And A Half Lim Domains Protein 2; Fhl-2; Lim Domain Protein Dral; Skeletal Muscle Lim-Protein 3; Slim-3 (Gene ID:2274; UniProt:Q14192)
    
    * For research use only
- Applications
  - Application
    
    ELISA; WB; IF; Flow Cytometry; IHC
    
    Application Description
    
    ELISA-antibody detection limit dilution 1:32000.
    WB-Approx 19kDa band observed in nuckear lysates of cell line HEK293, approx. 18kDa in nuckear lysates of cell line HeLa, and approx 17kDa in nuckear lysates of cell line NIH3T3. Recommended concentration: 0.3-1µg/ml. This molecular weight is routinely observed by other sources. Primary incubation 1 hour at room temperature.
    IHC-Paraffin embedded Human Pancreas and Brain. Recommended concentration: 3.75µg/ml.
    IF-Strong expression of the protein seen in the nuclei of A431 and HeLa cells. Recommended concentration: 10µg/ml.
    FC-Flow cytometric analysis of A431 cells. Recommended concentration: 10ug/ml.